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Bacteriological examination of urine

Known as: urine culture, uroculture
SKU: 5013

58.50

Study material: Urine
Response time (working day): 3
The test is done on an empty stomach: no
Home call service: Yes
Country: Georgia

General Information

Urine is produced by the kidneys to expel some impurities and excess substances from the body. After production, urine travels from the kidneys to the ureters (together they form the upper urinary tract), from where it passes into the bladder and is excreted from the body through the urethra (together they form the lower urinary tract).

Normally, the urinary tract is sterile, except for the distal urethra, which is colonized with bacteria from the skin of the perineum and in women from the vulva.

Urine Culture (Uroculture) Is an analysis in which material from a urine sample is sown on bacteriological soil to detect and identify the bacteria that cause Urine Of roads Infections (Sgi).

When should we take the test?

The test is prescribed by a doctor to diagnose urinary tract infections, especially in patients at risk for complications whose disease has been prolonged and who do not respond to first-line treatment. Based on the results, the doctor should select the most suitable (causing the least side effects) antibiotic to treat and prevent complications. Then it is necessary to constantly monitor the effectiveness of therapy.

The test should be performed in conjunction with general, microscopic, and biochemical tests of urine, which in turn can further aid in the diagnostics of urinary tract infections (e.g., detection of changes in urine pH, presence of leukocytes, increase in leukocytesterase activity, and nitrites).

If urosepsis (sepsis caused by the spread of infection from the urinary tract) is suspected, a blood culture test should be added to the urine culture.

A urine culture test is also performed prophylactically in pregnant women because in their case the bacteria found in the urine, even without symptoms of infection (asymptomatic bacterial), can spread to the upper urinary tract in the later stages of pregnancy and cause pyelonephritis.

who Should To conduct test?

People with symptoms of lower urinary tract infections:

  • Strong, insatiable desire to urinate
  • The feeling that urine is still in the bladder as soon as it is excreted
  • Pain, burning sensation when urinating
  • Change in urine odor and color
  • Pain in the lower abdomen

People with symptoms of upper urinary tract infections:

  • Pain in the lumbar region
  • Discomfort in the kidneys
  • Chills and fever
  • Nausea and vomiting
  • Hematuria (bloody urine)

Possible interpretation of the results

Negative - No bacteriological growth was observed within 48 hours.

  1. If bacteria are not detected in the urine culture but leukocytosis (increased concentration of leukocytes in the urine) is observed, this may indicate an infection causing the microorganisms to be difficult to cultivate (chlamydia, mycoplasma, Trichomonas, mycobacterium) or antibacterial.
  2. If symptoms persist despite a negative culture, non-infectious (non-bacterial) causes of urinary tract disease should also be considered.

Positive - If bacteria grow / multiply in the urine sample. A properly collected urine sample containing more than 100 colony-forming units (KTE / ml) of one type of bacterium usually indicates the presence of infection. The type of bacteria is then determined and an antibioticogram is set up (to determine the susceptibility of the bacteria to the antibiotic).

Sometimes more than one type of bacteria is found in the urine. Rarely, it is caused by an infection associated with more than one pathogen; However, it is more likely that the test sample was collected incorrectly and was contaminated with skin, vaginal or fecal bacteria. Mixed flora - which indicates contamination when taking material. The number of bacteria detected and their concentration are not determined. Bacteria are not identified.

Additional information

Urinary tract infections are caused by a variety of microorganisms that reach the urinary tract where they multiply and over time cause changes in the normal functioning of the kidneys and urinary tract.

According to localization sg It can be "lower" when the urethra and bladder are damaged and "upper" when the kidneys and ureters are damaged.

sg A fairly common problem, most infections affect the lower urinary tract (bladder), which is usually treated. However, if we do not detect the infection early enough, it can spread through the urethra, upwards, to the kidneys, causing dangerous pyelonephritis that damages the kidneys. In the most severe cases, the bacteria can spread into the bloodstream and cause life-threatening sepsis.

Depending on the symptoms, STDs may be asymptomatic - bacterial (bacteria in the urine) without clinical symptoms, and symptomatic - when the bacteriuria is accompanied by an inflammatory reaction (leukocytes in the urine) and clinical manifestations.

The incidence of urinary tract infections varies by sex and age: it is more common in boys in the first year of life (the result of phimosis and paraphimosis at this age), then the ratio equals in early childhood, followed by female dominance due to the female urethra being shorter and closer. Is near the anus, allowing intestinal bacteria to move more easily and colonize the urethra. The incidence of infections increases with age (associated with sexual activity and pregnancy). However, the difference between the sexes disappears in the elderly, with an increase in prevalence in men due to prostate diseases.

Patients with kidney (e.g., kidney stones) and other chronic diseases (e.g., diabetes), immunocompromised, or permanently catheterized individuals are at risk for developing recurrent STDs and complications.

In the elderly, immunocompromised patients, and children, the infection may be asymptomatic or nonspecific (fever, indigestion, weight loss).

Urine Of roads Infections In etiology Most Spread There are microbes:

  • Gram-negative Sticks (Escherichia coli ,, Klebsiella spp., Proteus spp., Morganella spp., Pseudomonas spp., Acinetobacter spp., Enterobacter spp, Providencia spp.).
  • Gram-positive Cokes (Enterococcus spp., Staphylococcus aureus, Staphylococcus saprophyticus, Group B hemolytic streptococci).
  • Corynebacterium urealisticum
  • Candida albicans Causes urethrocystitis in patients with diabetes mellitus.

Correct microbiological diagnostics requires clinical information about the patient (age, sex, pregnancy, antibiotic therapy, history of other urological diseases…).

How Get ready For the test / Of the material Collection rules

Bacteriological examination of urine should be performed:

  • Before treatment with antibacterial and antifungal agents.
  • No earlier than 7-10 days after the end of the course of treatment with antibacterial and antifungal drugs.
  • Against the background of antibacterial and antifungal treatment as prescribed by a physician.
  • The first morning urine should be collected for analysis or, if this is not possible, urine should be collected at least 4 hours after previous urination.
  • To collect the material, rinse the periurethral area with laundry soap and water or wipe with a disinfectant wipe, then pat dry with a clean, dry towel.
  • The first portion of urine should be drained and the middle portion of urine containing at least 10-20 ml should be placed in a sterile urine container. Do not collect the last portion of urine!
  • Do not fill the container to the brim!
  • Do not touch the inner walls of the container!
  • In infants - After washing the periurethral area with washing soap and water or cleaning with a disinfectant wipe, drying with a dry towel, it is advisable to collect urine in the urethra. Exhausted urine from diapers is impossible. Transfer urine from the ureter to a sterile container and bring to the laboratory.
  • Urine should be brought to the laboratory within one hour of ingestion. If you can not bring the test material to the laboratory within one hour, then it should be stored immediately in the refrigerator at a temperature of + 2–8 ° С for a maximum of 2 hours.
  • If the urine is placed in a sterile container containing a preservative (eg boric acid), the urine can be stored in the refrigerator for 24 hours.

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