Trichomonas vaginalis DNA (PJR in real time)

T. vaginalis reveals tropism in the vaginal and urethral mucosa in women and in the urethra in men, so there are clinical manifestations in the acute course Vaginitis, cervicitis and urethritis. Asymptomatic transmission of trichomoniasis, which contributes to the development of complications due to lack of treatment, is typical for 10-50% of women and 60-70% of men. T. vaginalis can cause complications such as premature rupture of membranes, premature birth, low fetal weight, infertility, and damage to appendages in men. It should be noted that T. vaginalis is associated with an increased risk of developing other sexually transmitted infections, especially Neisseria gonorrhoeae, so T. vaginalis infection should be ruled out in all patients diagnosed with gonorrhea.

Trichomoniasis is often asymptomatic and classic manifestations are found in a small percentage of infected people - foamy discharge is found in only 10% of patients with trichomoniasis, "strawberry cervix" - in 2% of cases and an increase in pH - in 16%. Thus, laboratory diagnostics and not the clinical picture play a crucial role in the diagnostics of trichomoniasis.

Laboratory diagnostics of diseases caused by Trichomonas vaginalis includes direct detection methods such as microscopic examination of stained smear, culture method by inoculation with special media, PCR diagnostics (detection of pathogen DNA) and cytological examination (PAP); Indirect method - detection of serological markers (IgG, IgM).

Currently the most common method of diagnosing trichomoniasis remains the method of molecular diagnostics - Polymerase chain reaction (PCR). This method allows the identification and appointment of treatment for N. gonorrhoeae in a short time with high diagnostic sensitivity (95-100%) and specificity (95-100%).

Research material

Recommended for PCR diagnostics:

• In men The first part of the morning urine is using. This is the most optimal material in which case the diagnostic sensitivity reaches 85% -95%.
• Consumption of the first part of urine in women has a lower diagnostic sensitivity (about 80-90%) than in men, so in the case of women Urogenital smear Recognized as the most optimal material for testing. The study of the first part of the urine in women can be used in situations where it is difficult to take a smear from the vagina and cervix (for example, in pregnant women) or in the face of a cystitis clinic (including chronic). It is the most optimal time to examine women The fourth week from the first day of the last menstrual period.
• You can pick up a smear brush and Eppendorf with transport fluid at Synevo branches.
• To take a smear, contact a doctor-specialist (urologist, gynecologist).

The research material should be brought to the laboratory by 13:00.

How to prepare for the test?

Before taking smear:

• Do not use a vaginal shower;
• It is recommended to abstain from sexual intercourse for at least 24-48 hours;
• Do not urinate directly before smearing;
• Contraceptive use should be discontinued three days in advance;
• The smear is taken 24-48 hours after the colposcopy and 24 hours after the vaginal ultrasound examination;

• Perform external genital hygiene procedures without the use of soap and other detergents;
• The test may be performed no earlier than 10-14 days after completion of antibacterial, antifungal, or topical antiseptics;
• It is recommended to perform the test during ovulation or in the presence of symptoms of inflammatory disease of the urogenital tract.
• Examination isn't performing during menstrual bleeding.
• The examination is performing 2 weeks after taking preparations containing microorganisms (probiotics, eubiotoc).