Trichomonas vaginalis DNA (PJR in real time)

Reference values

Trichomonas vaginalis exhibits tropism towards the vaginal and urethral mucosa in women, and towards the urethra in men, therefore, in the acute course, clinical manifestations are Vaginitis, cervicitis and urethritis.Asymptomatic transmission of trichomoniasis, which contributes to the development of complications due to the lack of treatment, is characteristic of 10-50% of women and 60-70% of men. T. vaginalis can cause complications such as premature rupture of membranes, premature birth, low birth weight, infertility, and damage to the appendages in men. It should be noted that T. vaginalis is associated with an increased risk of developing other sexually transmitted infections, especially Neisseria gonorrhoeae, therefore infection with Trichomonas vaginalis should be excluded in all patients with a diagnostics of gonorrhea.

Trichomoniasis is often asymptomatic and classic manifestations are found in a small percentage of infected people - foamy discharge is found in only 10% of patients with trichomoniasis, "strawberry cervix" - in 2% of cases and an increase in pH - in 16%. Thus, laboratory diagnostics and not the clinical picture play a crucial role in the diagnostics of trichomoniasis.

Laboratory diagnostics of diseases caused by Trichomonas vaginalis includes direct detection methods such as microscopic examination of stained smear, culture method by inoculation with special media, PCR diagnostics (detection of pathogen DNA) and cytological examination (PAP); Indirect method - detection of serological markers (IgG, IgM).

Currently the most common method of diagnosing trichomoniasis remains the method of molecular diagnostics - Polymerase chain reaction (PCR). This method allows the identification and appointment of treatment for N. gonorrhoeae in a short time with high diagnostic sensitivity (95-100%) and specificity (95-100%).

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