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The test is used to diagnose infection,
Western blotting Immunoblotting is a method that detects antibodies against a specific parasite protein.
Used as a confirmatory test, especially when ELISA is positive or doubtful
It is characterized by high specificity — reducing the risk of false-positive results, which are often found in ELISA due to cross-reaction with other parasites.
Strongyloides stercoralis It is a parasitic worm that causes strongyloidiasis.
The infection is often asymptomatic, but can cause chronic diarrhea, abdominal pain, skin rash, and cough.
In immunosuppressed patients (steroid therapy, transplantation), the infection can spread throughout the body, sometimes creating a life-threatening condition.
For confirmation — When the ELISA result is positive or equivocal
In immunosuppressed patients — When the antibody level is unreliable
Positive result – indicates the presence of Strongyloides-specific antibodies → confirmation of infection
Negative result – rules out strongyloidiasis, although it is important to consider the clinical picture
Ambiguous indicator – is the result of cross-reactivity of antigens with other pathogens and requires additional research methods
The Western blot method is the “gold standard” for confirming Strongyloides antibodies because it accurately identifies parasite-specific antigens and reduces the risk of false-positive results.
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