Dysbacteriosis Laboratory research

There is a clinical syndrome associated with dysbacteriosis characterized by diarrhea, steatorrhea, weight loss, and anemia. With a sharp weakening of the protective mechanisms, endogenous infection can be complicated by a generalized form such as sepsis.

From a microbiological point of view, there are usually three degrees of dysbiosis in the large intestine: I quality - Slight decrease in the number of bifidobacteria and / or lactobacilli, increase / decrease in the content of intestinal sticks, the appearance of small titers of altered forms (with hemolytic properties (hem (+)) or lactose-negative (lac (-)) strains); II quality - Existence of one species of opportunistic (so-called conditionally-pathogenic) microorganisms with a concentration of not more than 10⁵ kcse / g (colony forming unit / g) or their associations in small titers (E. coli lac (-), E. coli hem (+), Proteus spp., Clostridium spp., Klebsiella spp., Pseudomonas spp., Acinetobacter spp.); III quality - High titer of conditionally pathogenic microorganisms, both in the same species and in the form of associations.

Healthy human's Intestine Microflora Composition:

• Intestinal sticks (E. coli) With normal properties;
• Lactose-negative intestinal sticks (E. coli);
• Conditionally pathogenic microflora (Proteus spp., Klebsiella spp., Enterobacter spp., Citrobacter spp. etc. Non-fermenting bacteria such as Pseudomonas spp., Acinetobacter spp.);
• Enterococci;
• Non-pathogenic staphylococci;
• Bifidobacteria;
• Lactobacilli (lactic acid bacilli);
• Mushroom Candida (Candida spp.);
• Hemolytic microorganisms (hemolytic intestinal sticks (E. coli hem (+)), hemolytic enterococci, Staphylococcus aureus (S. aureus)) Is not usually detected;
• Pathogenic enterobacteria are not usually detected.

Even the detection of pathogenic microorganisms in the feces does not always indicate the presence of disease. Therefore, evaluation of results should be performed in the light of clinical data.

material Material/should Collection Rule

Research material - Feces

For bacteriological examination material should be taken:

• Before treatment with antibacterial and antifungal agents.
• No earlier than 7-10 days after the end of the course of treatment with antibacterial and antifungal drugs.
• Against the background of antibacterial and antifungal treatment as prescribed by a physician.

• Bacteriological examination of feces does not require special preparation.
• The test material is collected in a sterile container with a screw cap and a with spoon on the lid
• for examination, feces should not contain impurities such as urine, so you should urinate before collecting feces to exclude the possibility of entering urine in the feces.
• Stools are collected from a sterile surface - the so-called. Night pot or "Sudno", which is well washed with soap, rinsed several times with water, and then treated with boiling water and cooled. It is forbidden to treat the surface of the pot with disinfectants.
• The material for examination is taken mainly from the middle portion of feces, from 4-5 different places, with a special spoon fixed on the lid of the sterile container, in an amount not more than 1/3 of the container volume (up to 10 grams - 1 spoon)
• Do not fill the container to the brim!
• After collecting the feces in the container, close the lid of the container tightly and bring the material to the laboratory center.

The study material should be brought to the laboratory within one hour of collection. If you can not bring the test material to the laboratory within one hour, then it should be stored immediately in the refrigerator at a temperature of + 2–8 ° С for a maximum of 2 hours.