Active vitamin B12 (holotranscobalamin) | Laboratory research

Cyanocobalamin is essential for the normal maturation of erythrocytes, it acts as a coenzyme in the synthesis of nucleic acids - DNA and RNA, and methionine. Methionine is essential for the synthesis of folic acid, which provides the normoblastic type of hematopoiesis.

Cyanocobalamin provides the synthesis of lipoproteins in myelin tissue as well as the synthesis of glutathione (hence its deficiency is accompanied by the development of pernicious anemia, neutropenia and neurological disorders).

A specific protein transcobalamin transports cobalamins in the blood plasma. The transcobalamin-cobalamin complex is called holotranscobalamin (HoloTC) or active vitamin B12. HoloTC makes up only 12% of circulating vitamin B25 in the blood, yet is the only form of vitamin B12 that can be absorbed by the body's cells.

The definition of holotranscobalamin (active vitamin B12) has several advantages:

• The first early marker indicating early deficiency of vitamin B12 due to a short half-life;
• contains biologically active vitamin B12;
• Low levels of active vitamin B12 indicate a deficiency of vitamin B12, although total vitamin B12 levels may be within the normal range;

Vitamin B12 is not produced in the body and therefore should be taken with food. Vitamin is contained in products of animal origin, e.g. Milk, cheese, eggs, meat, mussels and crustaceans.

Vitamin B12 is absorbed in the distal part of the intestine of the thigh, in this case the precondition of absorption is its connection with an internal factor - glycoprotein secreted by the stomach.

Absorption of vitamin B12 usually depends on: 1) the secretion of endogenous factor in the stomach; 2) the integrity of the distal intestinal mucosa; 3) the presence of a sufficient amount of transcobalamin in plasma; 4) the condition of the intestinal microflora (some bacteria may interfere with absorption, as the vitamin is essential for their vital activity). Insufficient absorption in the intestine is a major cause of vitamin B12 deficiency.

Insufficient intake of vitamin B12 from food occurs in the elderly and vegetarians.

Relative deficiencies of vitamin B12 and folic acid may develop during pregnancy due to the body's increased need for these vitamins.

Vitamin B12 deficiency, without treatment, can lead to anemia, fatigue, muscle weakness, intestinal and stomach problems, nerve damage, and changes in mood and cognitive function.

How should we prepare for the test?

Preferably for the test on empty stomach Presence.

The test is recommended before a possible injection of vitamin B12.

It is recommended to limit alcohol intake, smoking and strenuous physical activity for at least 12 hours before the test.