Determining cortisol in saliva is a simple and non-invasive method for examining the level of the stress hormone.
Why saliva? 
– The sample collection process is simple and non-invasive
– It is possible to collect several samples at different times of the day
– Salivary cortisol is less susceptible to stress-induced spikes
– Free cortisol is measured in saliva, not protein-bound cortisol
– Useful for studying sleep and stress (taking into account circadian rhythms)
What is it used for? 
Circadian rhythm and sleep disorders
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